1. Ang methyl acetoacetate ay isang intermediate ng fungicides, tulad ng oxadiazinol, dimethylazoxyphenol, acetaminophen, insecticides, tulad ng diazinon, phoxim, pyrimidin, herbicide imazethapyranoic acid, rodenticides, warfarin, warfarin, atbp.
2. Ginagamit ito bilang bahagi ng cellulose ether ester mixed solvent, at ginagamit din sa organic synthesis ng gamot, dye, pigment, molecular stabilizer, atbp.